What is a 3-part differential hematology analyzer
3-part differential hematology analyzer which analyzes the complete blood count technology wise.
Principle of Measurement –
1 . Impedance Principle for RBC/PLT/WBC Differential
2. Colorimetry method for HGB.
What is Impedance principle
When blood cells enter the narrow aperture of electrodes which generates the Signal every time when cell passes through, signals are nothing, but the electrodes generate a resistance within the aperture when the cell pass through it which causes an Impedance and these impedances are transferred as a signal and count to measure the volume of cells.
Since the WBC lysing agent lyses the Cell membrane of the WBC but not the nucleus, the Monocytes, get into the Mid-cells along with the Basophil & the Eosinophil.
The Neutrophils, being polymorphous, have multiple nuclei and show up at the largest size in the Histogram.
Colorimetric:
It is a photometric technique which states that coloured compounds can absorb a certain wavelength of light when monochromatic light is passed through them. The working of a colorimeter is based on the concept of Beer-Lambert’s law.
It’s nothing but The amount of light absorbed is directly proportional to the concentration of the solution.
Output results in 3-Part Hematology Analyzer parameters are:
WBC – Total WBC count , Lymph%/#, Mid %/#, Gran%/#, NLR
RBC – Total RBC count ,HGB, HCT, MCV MCH, MCHC, RDW-CV, RDW-SD,
PLT – Total Platelet count, MPV, PCT, PDW-CV, PDW-SD, P-LCR, P-LCC, PLR
Supporting Consumables to run the System:
Two reagents + 1 cleaner play important role to run the 3-Part Analyzer, they are ,
Diluent – To dilute blood samples, to keep the blood cell in original volume also provides appropriate conductivity to the cells.
Lyse – Lysing Reagents are used to rupture the RBC to measure Hemoglobin, prepare WBC from whole blood to measure the differentials
Probe Cleaner – To clean the instrument’s probe or tubes. It can also be used for daily maintenance.